recombinant stc 1 protein  (Sino Biological)

Journal: Bioactive Materials

Article Title: Mossy-textured hydroxyapatite-modified poly (lactic-co-glycolic acid) microspheres promote collagen regeneration via calcium/TGF-β and chemokine signaling pathways in soft tissue augmentation

doi: 10.1016/j.bioactmat.2025.12.028

Figure Lengend Snippet: The regulatory effects of CaHA/PLGA microspheres on macrophages and ADSCs in vitro. (A, B) CLSM images and RFI of CD86 and CD206 expression in RAW264.7 cells co-cultured with microspheres for 2 days (n = 3). (C–F) Relative mRNA expression levels of inflammation-related genes TNF-α, IL-6, TGF-β1, and FGF-2 in RAW264.7 cells (n = 3). (G, H) Sirius red staining images and quantitative analysis (n = 3) of collagen deposition of ADSCs co-cultured with CaHA/PLGA microspheres for 3 and 7 days. (I–K) Relative mRNA expression levels of TGF-β1, FGF-2, and PDGF-A in ADSCs after 3 and 7 days of co-culture with CaHA/PLGA microspheres (n = 3). ∗ p < 0.05; ∗∗ p < 0.01; ∗∗∗ p < 0.001; ∗∗∗∗ p < 0.0001; ns , not significant.

Article Snippet: CD86 polyclonal antibody (1:200, 13395-1-AP, Proteintech), CD206 (1:200, 18704-1-AP, Proteintech), and TNF-α recombinant antibody (1:200, 80258-6-RR, Proteintech) were used as primary antibodies, incubated overnight at 4 °C.

Techniques: In Vitro, Expressing, Cell Culture, Staining, Co-Culture Assay

Journal: Bioactive Materials

Article Title: Mossy-textured hydroxyapatite-modified poly (lactic-co-glycolic acid) microspheres promote collagen regeneration via calcium/TGF-β and chemokine signaling pathways in soft tissue augmentation

doi: 10.1016/j.bioactmat.2025.12.028

Figure Lengend Snippet: Evaluation of soft tissue filling and inflammatory response in rats. (A, B) Schematic diagram of the soft tissue filling experiment and injection sites, with at least 2 cm spacing between sites. (C) Photographs of subcutaneous soft tissue filling at 2, 4, 8, and 12 weeks. The white circles indicate the soft tissue filling areas. (D) H&E staining of the filled sites. (E, F) Immunofluorescence images and RFI of TNF-α and TGF-β expression at 2 weeks post-filling (n = 6). (G, H) Immunofluorescence images and RFI of CD86 and CD206 expression at 2 weeks post-filling (n = 6). ∗ p < 0.05; ∗∗ p < 0.01; ∗∗∗ p < 0.001; ∗∗∗∗ p < 0.0001; ns , not significant.

Article Snippet: CD86 polyclonal antibody (1:200, 13395-1-AP, Proteintech), CD206 (1:200, 18704-1-AP, Proteintech), and TNF-α recombinant antibody (1:200, 80258-6-RR, Proteintech) were used as primary antibodies, incubated overnight at 4 °C.

Techniques: Injection, Staining, Immunofluorescence, Expressing

Journal: Experimental and Therapeutic Medicine

Article Title: Appendiceal mucinous tumour resulting in autoamputation of the appendix: A case report and literature review

doi: 10.3892/etm.2025.13043

Figure Lengend Snippet: Immunohistochemical markers in the removed appendix tissue. (A) CKpan positivity; (B) CK7 negativity; (C) CK20 positivity; (D) caudal type homeobox 2 positivity; (E) MUC2 positivity; (F) MUC5AC negativity; (G) PAX-2 negativity; (H) PAX-8 negativity; and (I) Ki-67 negativity. CK, cytokeratin; PAX, paired box; MUC, mucin.

Article Snippet: Primary antibody details were as follows: CK-pan mouse monoclonal antibody (7H8C4; 1:200; cat. no. EM1712-42; HUABIO), CK7 recombinant monoclonal antibody (1:2,000; cat. no. 86153-7-RR; Proteintech Group, Inc.), CK20 recombinant monoclonal antibody (1:4,000; cat. no. 82428-1-RR; Proteintech Group, Inc.), CDX2 recombinant monoclonal antibody (1:1,000; cat. no. 82659-1-RR; Proteintech Group, Inc.), MUC2 polyclonal antibody (1:2,000; cat. no. 27675-1-AP; Proteintech Group, Inc.), MUC5AC polyclonal antibody (1:500; cat. no. 20725-1-AP; Proteintech Group, Inc.), PAX2 polyclonal antibody (1:1,000; cat. no. 29307-1-AP; Proteintech Group, Inc.), PAX8 polyclonal antibody (1:800; cat. no. 10336-1-AP; Proteintech Group, Inc.) and Ki-67 polyclonal antibody (1:4,000; cat. no. 27309-1-AP; Proteintech Group, Inc.).

Techniques: Immunohistochemical staining